thr77 Secrets
thr77 Secrets
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in many tumors, which results in chromosome instability, and thus might contribute to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential position in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two more splice variants have been noted but comprehensive nucleotide sequence information is not really still offered.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide typically is made up of the epitope regarded from the antibody.
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This guide outlines further probable optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guidebook outlines more probable optimization of cellular and immunoassay parameters to be sure the best possible results are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs into the really conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is also degraded as cells development by S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the thr77 login modulation of receptor activation by equally antibodies...
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